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- Title
Widespread expression of parathyroid hormone-related peptide in melanocytic cells.
- Authors
Kageshita, T.; Ishihara, T.; Tokuo, H.; Funasaka, Y.; Ichihashi, M.; Dong, J.; Nakajima, M.; Ono, T.
- Abstract
Summary Background Parathyroid hormone-related peptide (PTH-rP) was associated with the syndrome of hypercalcaemia of malignancy. An increased serum level of PTH-rP could occur in patients with advanced melanoma. Objectives We examined PTH-rP expression in cultured melanocytic cell lines and in lesions of melanocytic origin for associations with clinicopathological variables of disease progression. We measured the supernatant and cell lysate level of PTH-rP in cultured melanoma cells to clarify whether melanoma cells secrete PTH-rP. Methods PTH-rP expression was examined by reverse transcriptase–polymerase chain reaction (RT–PCR) in cultured melanocytic cell lines and by immunoperoxidase staining in 18 melanocytic naevi, 40 primary melanoma and 19 metastatic melanoma lesions. The supernatant level of PTH-rP was measured with an immunoradiometric assay. Results RT–PCR products of PTH-rP mRNA were detected in six of eight melanoma cell lines; however, neither naevus cells nor melanocytes showed positive products. On the other hand, immunohistochemical analysis showed that PTH-rP was widely expressed both in benign and malignant melanocytic lesions. In addition, PTH-rP expression was not associated with any clinicopathological variables. Cell lysate but not the supernatant of melanoma cells showed high PTH-rP levels. Conclusions These results suggest that PTH-rP was widely expressed in melanocytic cells; however, the cells did not secrete PTH-rP.
- Subjects
CELL lines; PEPTIDES
- Publication
British Journal of Dermatology, 2003, Vol 148, Issue 3, p533
- ISSN
0007-0963
- Publication type
Article
- DOI
10.1046/j.1365-2133.2003.05171.x