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- Title
The role of circulating food antigen-specific lymphocytes in food allergic children with atopic dermatitis.
- Authors
Reekers, R.; Beyer, K.; Niggemann, B.; Wahn, U.; Freihorst, J.; Kapp, A.; Werfel, T.
- Abstract
In this study we evaluated antigen-specific <em>in vitro</em> responses of peripheral blood lymphocytes to lipopolysaccharide (LPS)-depleted food allergens in children who reacted to food challenge (cow's milk or hen's egg) with a deterioration of their atopic dermatitis (AD). Some of the children showed immediate symptoms (urticaria. bronchial asthma or gastrointestinal symptoms) as well. The proliferation of casein-stimulated lymphocytes from children reacting to cow's milk (age 0.7-5.9 years) was significantly higher (P < 0.01) than the proliferation of lymphocytes from 15 children with AD without milk allergy (age: 2.1-9.1 years). Twenty-eight T-cell clones (TCC) were established from the blood of three children sensitized to cow's milk and hen's egg who reacted to double-blind, placebo-controlled oral food challenge both with a deterioration of AD and with immediate symptoms. Surprisingly. 16 of 28 casein- or ovalbumin-specific TCC were CD8+. All TCC produced high amounts of IFN-γ upon stimulation with concanavalin A. in addition, 75% of the CD4+ TCC and 44% of the CD8+ TCC secreted IL-4. Our results indicate that: (i) food-specific proliferation of blood lymphocytes can be detected in patients with clinically relevant food allergy with LPS-depleted allergens <em>in vitro</em> and (ii) circulating food-specific lymphocytes are CD4+ and CD8+. T cells with the capacity of producing both type 1 and type 2 cytokines.
- Subjects
ANTIGENS; ATOPIC dermatitis; LYMPHOCYTES; ALLERGY in children; T cells; IMMUNOREGULATION
- Publication
British Journal of Dermatology, 1996, Vol 135, Issue 6, p935
- ISSN
0007-0963
- Publication type
Article
- DOI
10.1046/j.1365-2133.1996.d01-1098.x