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- Title
Structural insight into catalytic mechanism of PET hydrolase.
- Authors
Xu Han; Weidong Liu; Jian-Wen Huang; Jiantao Ma; Yingying Zheng; Tzu-Ping Koa; Limin Xu; Ya-Shan Cheng; Chun-Chi Chen; Rey-Ting Guo
- Abstract
PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.
- Subjects
POLYETHYLENE terephthalate; BIOCONVERSION; PLASTICS; CATALYSIS; MUTAGENESIS
- Publication
Nature Communications, 2017, Vol 8, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-017-02255-z