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- Title
Bacterial Outer Membrane Vesicles as Nano‐Scale Bioreactors: A Fatty Acid Conversion Case Study.
- Authors
Song, Ji‐Won; Baeg, Yoonjin; Jeong, Ha‐Yeon; Lee, Jinwon; Oh, Deok‐Kun; Hollmann, Frank; Park, Jin‐Byung
- Abstract
Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, involved in various functions including cell‐to‐cell signalling and protein excretion. We have engineered the OMVs of Escherichia coli to nano‐scaled bioreactors for the biotransformation of fatty acids by targeting a fatty acid double bond hydratase of Stentrophomonas maltophilia (SmOhyA) and/or a photoactivated fatty acid decarboxylase from Chlorella variabilis NC64 A (CvFAP) into OMVs. Engineered OMVs containing both SmOhyA and CvFAP were able to catalyse the transformation of oleic acid ((Z)‐octadec‐9‐enoic acid) into 9‐hydroxyheptadecane via (R)‐10‐hydroxyoctadecanoic acid. The specific biotransformation rates of oleic acid reached 8.0×10−12 μmol/min per OMV.
- Subjects
EXTRACELLULAR vesicles; FATTY acids; BIOREACTORS; DOUBLE bonds; BIOCONVERSION; BACTERIAL cell walls
- Publication
ChemCatChem, 2021, Vol 13, Issue 19, p4080
- ISSN
1867-3880
- Publication type
Article
- DOI
10.1002/cctc.202100778