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- Title
Genetic characterization of a novel blaDIM-2-carrying megaplasmid p12969-DIM from clinical Pseudomonas putida.
- Authors
Fengjun Sun; Dongsheng Zhou; Qian Wang; Jiao Feng; Wei Feng; Wenbo Luo; Yao Liu; Xuewen Qiu; Zhe Yin; Peiyuan Xia; Sun, Fengjun; Zhou, Dongsheng; Wang, Qian; Feng, Jiao; Feng, Wei; Luo, Wenbo; Liu, Yao; Qiu, Xuewen; Yin, Zhe; Xia, Peiyuan
- Abstract
<bold>Objectives: </bold>To characterize a blaDIM-2-carrying 409 kb megaplasmid p12969-DIM of Pseudomonas putida 12969 from a patient with pneumonia in China.<bold>Methods: </bold>The complete nucleotide sequence of p12969-DIM was determined with a paired-end library and a mate-pair library using next-generation sequencing technology.<bold>Results: </bold>blaDIM-2, a close blaDIM-1 variant, was identified in p12969-DIM. DIM-2 differs from DIM-1 by two amino acid substitutions Ser119Leu and Ser209Pro. The p12969-DIM backbone is highly similar to pOZ176, but the IncP-2-type stability/replication/conjugal transfer system in the pOZ176 backbone is absent from p12969-DIM. The p12969-DIM accessory regions, a 45.7 kb MDR and a novel insertion sequence, ISPpu23, are almost entirely distinct from pOZ176. The MDR region contains a novel Tn21-subgroup transposon Tn6286 inserted with two class 1 integrons, In1225 and In1226; a Tn5503-family transposon-like element inserted with a strAB locus; and a novel Tn21-subgroup transposon-like element inserted with a class 1 integron, In1224. The three integrons carry blaDIM-2 as well as a number of additional genes conferring resistance to quinolones, aminoglycosides, chloramphenicol, florfenicol, trimethoprim, streptomycin, quaternary ammonium compounds and sulphonamides. p12969-DIM has two distinct replication/stability systems, repA/parAB-parB2 of an unknown incompatibility group in the backbone and repABC/mazFE of the IncQ2 group in the MDR region.<bold>Conclusions: </bold>The MDR region of p12969-DIM harbours many resistance genes as well as a second replication/stability system. This article is the first report of a fully sequenced blaDIM-carrying plasmid.
- Subjects
PSEUDOMONAS putida; PLASMIDS; NUCLEOTIDE sequence; TRANSPOSONS; QUINOLONE antibacterial agents; AMINOGLYCOSIDES; CHLORAMPHENICOL; BACTERIA; AMINO acids; ANTIBIOTICS; COMPARATIVE studies; DRUG resistance in microorganisms; GENES; HYDROLASES; RESEARCH methodology; MEDICAL cooperation; MICROBIAL sensitivity tests; PSEUDOMONAS; PSEUDOMONAS diseases; RESEARCH; EVALUATION research; PHARMACODYNAMICS
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2016, Vol 71, Issue 4, p909
- ISSN
0305-7453
- Publication type
journal article
- DOI
10.1093/jac/dkv426