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- Title
Pathogenicity of pan-drug-resistant Serratia marcescens harbouring bla<sub>NDM-1</sub>.
- Authors
Grubest, Teresa M.; Göttig, Stephan; Mark, Laura; Christ, Sara; Kempf, Volkhard A. J.; Wichelhaus, Thomas A.; Hamprecht, Axel
- Abstract
Objectives: To characterize a pan-drug-resistant Serratia marcescens clinical isolate carrying the New Delhi metallo-β-lactamase (NDM)-1. Methods: The presence of β-lactamase genes was examined by PCR and sequencing. Antibiotic susceptibility was determined by antibiotic gradient test. Transformation assays, transconjugation assays, PFGE and PCRbased replicon typing were used for plasmid analysis. Horizontal gene transfer was evaluated by liquid mating using Escherichia coli J53 as a recipient. Pathogenicity of NDM-1 expressing S. marcescens was analysed using the Galleria mellonella infection model. Results: S. marcescens isolate SM1890 was non-susceptible to all tested antibiotics, with minocycline retaining intermediate activity. blaNDM-1 was located on a 140 kb IncA/C-type plasmid which was transferable to E. coli and Klebsiella pneumoniae by conjugation. The LD50 of the NDM-positive, SM1890 isolate was higher than that of other, NDM-1 negative, S. marcescens strains. Conclusions: The presence of a blaNDM-1-harbouring IncA/C plasmid resulted in marked resistance to β-lactam antibiotics, but had no significant effect on virulence of isogenic strains. Because of the intrinsic resistance of S. marcescens to colistin and reduced susceptibility to tigecycline, treatment options for infections by NDM-1-positive isolates are extremely limited in this species.
- Subjects
DRUG resistance in bacteria; SERRATIA marcescens; BETA lactamases; GENETIC transformation; POLYMERASE chain reaction; MICROBIAL sensitivity tests; BACTERIA
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2015, Vol 70, Issue 4, p1026
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dku482