We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Noninvasive Allograft Imaging of Acute Rejection: Evaluation of I-anti-CXCL10 mAb.
- Authors
Cheng, Dayan; Sun, Hukui; Liang, Ting; Zhang, Chao; Song, Jing; Hou, Guihua
- Abstract
The purpose of this study was to investigate the use of iodine-131-labeled anti-CXCL10 mAb as tracer targeted at CXCL10 to detect acute rejection (AR) with mice model. Expression of CXCL10 was proved by RT-PCR, ELISA, and immunochemistry staining. All groups were submitted to whole-body autoradioimaging and ex vivo biodistribution studies after tail vein injection of I-anti-CXCL10 mAb. The highest concentration/expression of CXCL10 was detected in allograft tissue compared with allograft treated with tacrolimus and isograft control. Tacrolimus could obviously inhibit the rejection of allograft. Allograft could be obviously imaged at all checking points, much clearer than the other two groups. The biodistribution results showed the highest uptake of radiotracer in allograft. T/NT (target/nontarget) ratio was 4.15 ± 0.25 at 72 h, apparently different from allograft treated with tacrolimus (2.29 ± 0.10), P < 0.05. These data suggest that CXCL10 is a promising target for early stage AR imaging and I-CXCL10 mAb can successfully image AR and monitor the effect of immunosuppressant.
- Subjects
HOMOGRAFTS; GRAFT rejection; IODINE compounds; IMMUNOSUPPRESSIVE agents; NONINVASIVE diagnostic tests; DRUG utilization; ENZYME-linked immunosorbent assay
- Publication
Inflammation, 2015, Vol 38, Issue 1, p456
- ISSN
0360-3997
- Publication type
Article
- DOI
10.1007/s10753-014-0050-8