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- Title
β-Tricalcium Phosphate-Modified Aerogel Containing PVA/Chitosan Hybrid Nanospun Scaffolds for Bone Regeneration.
- Authors
Boda, Róbert; Lázár, István; Keczánné-Üveges, Andrea; Bakó, József; Tóth, Ferenc; Trencsényi, György; Kálmán-Szabó, Ibolya; Béresová, Monika; Sajtos, Zsófi; D. Tóth, Etelka; Deák, Ádám; Tóth, Adrienn; Horváth, Dóra; Gaál, Botond; Daróczi, Lajos; Dezső, Balázs; Ducza, László; Hegedűs, Csaba
- Abstract
Electrospinning has recently been recognized as a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds. The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions. The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si. The viability of dental pulp stem cells on the BTCP-AE-FM was proven by an alamarBlue assay and with a scanning electron microscope. Critical-size calvarial defects in rats were performed as in vivo experiments to investigate the influence of meshes on bone regeneration. PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months. New bone formations were confirmed by histological analysis. Despite a slight change in the morphology of the mesh because of cross-linking, the BTCP-AE-FM basically retained its fibrous, porous structure and hydrophilic and biocompatible character. Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
- Subjects
BONE regeneration; AEROGELS; CHITOSAN; POLYVINYL alcohol; TISSUE scaffolds; SCANNING electron microscopes
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 8, p7562
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms24087562