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- Title
Intrauterine L-NAME Exposure Weakens the Development of Sympathetic Innervation and Induces the Remodeling of Arterial Vessels in Two-Week-Old Rats.
- Authors
Selivanova, Ekaterina K.; Shvetsova, Anastasia A.; Borzykh, Anna A.; Gaynullina, Dina K.; Kiryukhina, Oxana O.; Lukoshkova, Elena V.; Potekhina, Viktoria M.; Kuzmin, Vladislav S.; Tarasova, Olga S.
- Abstract
Nitric oxide (NO) has been shown to stimulate differentiation and increase the survival of ganglionic sympathetic neurons. The proportion of neuronal NOS-immunoreactive sympathetic preganglionic neurons is particularly high in newborn rats and decreases with maturation. However, the role of NO in the development of vascular sympathetic innervation has never been studied before. We tested the hypothesis that intrauterine NO deficiency weakened the development of vascular sympathetic innervation and thereby changed the contractility of peripheral arteries and blood pressure level in two-week-old offspring. Pregnant rats consumed NOS inhibitor L-NAME (250 mg/L in drinking water) from gestational day 10 until delivery. Pups in the L-NAME group had a reduced body weight and blood level of NO metabolites at 1–2 postnatal days. Saphenous arteries from two-week-old L-NAME offspring demonstrated a lower density of sympathetic innervation, a smaller inner diameter, reduced maximal active force and decreased α-actin/β-actin mRNA expression ratio compared to the controls. Importantly, pups in the L-NAME group exhibited decreased blood pressure levels before, but not after, ganglionic blockade with chlorisondamine. In conclusion, intrauterine L-NAME exposure is followed by the impaired development of the sympathetic nervous system in early postnatal life, which is accompanied by the structural and functional remodeling of arterial blood vessels.
- Subjects
GENE expression; SYMPATHETIC nervous system; BLOOD pressure; INNERVATION; BLOOD vessels; RATS
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 22, p12327
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms222212327