We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Bacterial Small RNAs in the Genus Herbaspirillum spp.
- Authors
Carvalho Garcia, Amanda; dos Santos, Vera Lúcia Pereira; Santos Cavalcanti, Teresa Cristina; Collaço, Luiz Martins; Graf, Hans
- Abstract
The genus Herbaspirillum includes several strains isolated from different grasses. The identification of non-coding RNAs (ncRNAs) in the genus Herbaspirillum is an important stage studying the interaction of these molecules and the way they modulate physiological responses of different mechanisms, through RNA–RNA interaction or RNA–protein interaction. This interaction with their target occurs through the perfect pairing of short sequences (cis-encoded ncRNAs) or by the partial pairing of short sequences (trans-encoded ncRNAs). However, the companion Hfq can stabilize interactions in the trans-acting class. In addition, there are Riboswitches, located at the 5′ end of mRNA and less often at the 3′ end, which respond to environmental signals, high temperatures, or small binder molecules. Recently, CRISPR (clustered regularly interspaced palindromic repeats), in prokaryotes, have been described that consist of serial repeats of base sequences (spacer DNA) resulting from a previous exposure to exogenous plasmids or bacteriophages. We identified 285 ncRNAs in Herbaspirillum seropedicae (H. seropedicae) SmR1, expressed in different experimental conditions of RNA-seq material, classified as cis-encoded ncRNAs or trans-encoded ncRNAs and detected RNA riboswitch domains and CRISPR sequences. The results provide a better understanding of the participation of this type of RNA in the regulation of the metabolism of bacteria of the genus Herbaspirillum spp.
- Subjects
GRASSES; NON-coding RNA; MESSENGER RNA; METABOLISM; PROKARYOTES
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 1, p46
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20010046