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- Title
Mitochondria Synthesize Melatonin to Ameliorate Its Function and Improve Mice Oocyte's Quality under in Vitro Conditions.
- Authors
Changjiu He; Jing Wang; Zhenzhen Zhang; Minghui Yang; Yu Li; Xiuzhi Tian; Teng Ma; Jingli Tao; Kuanfeng Zhu; Yukun Song; Pengyun Ji; Guoshi Liu
- Abstract
The physiology of oocyte in vitro maturation remains elusive. Generally, the oocytes have a very low maturation rate under in vitro conditions. In the current study, we found that melatonin promotes the maturation of oocytes in which mitochondria play a pivotal role. It was identified that; (1) mitochondria are the major sites for melatonin synthesis in oocytes and they synthesize large amounts of melatonin during their maturation; (2) melatonin improves mitochondrial function by increased mtDNA copy, mitochondrial membrane potential (ΔΨm) and mitochondrial distribution and ATP production in oocytes; (3) the meiotic spindle assembly is enhanced; (4) melatonin reduces ROS production and inhibits 8-oxodG formation, thereby protecting potential DNA mutation from oxidative damage. As a result, melatonin improves the quality of oocytes, significantly accelerates the developmental ability of IVF embryo. The results provide novel knowledge on the physiology of oocyte's maturation, especially under in vitro conditions.
- Subjects
ANTIOXIDANTS; MELATONIN; MITOCHONDRIA; MITOCHONDRIAL membranes; DNA mutational analysis
- Publication
International Journal of Molecular Sciences, 2016, Vol 17, Issue 6, p939
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms17060939