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- Title
Enhanced tyrosine hydroxylase expression in PC12 cells co-cultured with feline mesenchymal stem cells.
- Authors
Guang-Zhen Jin; Xi-Jun Yin; Xian-Feng Yu; Su-Jin Cho; Hyo-Sang Lee; Hyo-Jong Lee; Il-Keun Kong
- Abstract
Mesenchymal stem cells (MSCs) secrete a variety of neuroregulatory molecules, such as nerve growth factor, brain-derived neurotrophic factor, and glial cell-derived neurotrophic factor, which upregulate tyrosine hydroxylase (TH) gene expression in PC12 cells. Enhancing TH gene expression is a critical step for treatment of Parkinson's disease (PD). The objective of this study was to assess the effects of co-culturing PC12 cells with MSCs from feline bone marrow on TH protein expression. We divided the study into three groups: an MSC group, a PC12 cell group, and the combined MSC + PC12 cell group (the co-culture group). All cells were cultured in DMEM-HG medium supplemented with 10% fetal bovine serum for three days. Thereafter, the cells were examined using western blot analysis and immunocytochemistry. In western blots, the co-culture group demonstrated a stronger signal at 60 kDa than the PC12 cell group (p < 0.001). TH was not expressed in the MSC group, either in western blot or immunocytochemistry. Thus, the MSCs of feline bone marrow can up-regulate TH expression in PC12 cells. This implies a new role for MSCs in the neurodegenerative disease process.
- Subjects
TYROSINE; GENE expression; MESENCHYME; STEM cells; NERVE growth factor; NEUROTROPHINS
- Publication
Journal of Veterinary Science, 2007, Vol 8, Issue 4, p377
- ISSN
1229-845X
- Publication type
Article
- DOI
10.4142/jvs.2007.8.4.377