We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Neuroprotective Effect of Phlorotannin Isolated from Ishige okamurae Against HO-Induced Oxidative Stress in Murine Hippocampal Neuronal Cells, HT22.
- Authors
Heo, Soo-Jin; Cha, Seon-Heui; Kim, Kil-Nam; Lee, Seung-Hong; Ahn, Ginnae; Kang, Do-Hyung; Oh, Chulhong; Choi, Young-Ung; Affan, Abu; Kim, Daekyung; Jeon, You-Jin
- Abstract
The present study is designed to investigate the neuroprotective effect of a kind of phlorotannins, diphlorethohydroxycarmalol (DPHC) isolated from Ishige okamurae against hydrogen peroxide (HO)-induced oxidative stress in murine hippocampal neuronal cells, HT22. HO treatment induced neurotoxicity, whereas DPHC prevented cells from HO-induced damage then restoring cell viability was significantly increased. DPHC slightly reduced the expression of Bax induced by HO but recovered the expression of Bcl-xL as well as caspase-9 and -3 mediated PARP cleavage by HO. Intracellular reactive oxygen species (ROS) and lipid peroxidation was overproduced as the result of the addition of HO; however, these ROS generations and lipid peroxidation were effectively inhibited by addition of DPHC in a dose-dependent manner. Moreover, DPHC suppressed the elevation of HO-induced Ca release. These findings indicate that DPHC has neuroprotective effects against HO-induced damage in neuronal cells, and that an inhibitory effect on ROS production may contribute to the underlying mechanisms.
- Subjects
PHLOROTANNINS; HYDROGEN peroxide; OXIDATIVE stress; CELLS; NEUROTOXICOLOGY
- Publication
Applied Biochemistry & Biotechnology, 2012, Vol 166, Issue 6, p1520
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-012-9545-7