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- Title
Inhibition of nitric oxide synthase does not influence urinary nitrite plus nitrate excretion after renal ischemic injury.
- Authors
Ogawa, Toshihisa; Nussler, Andreas K.; Tuzuner, Eda; Mimura, Yoshikazu; Kaminishi, Michio; Beger, Hans G.
- Abstract
Background and aims: Whether renal nitric oxide production caused by ischemia/reperfusion (I/R) influences the urinary excretion of nitric oxide (NO) metabolites (nitrite and nitrate) is far from being elucidated. In the present study, we evaluated the role of NO synthase inhibition using NG-nitro-L-arginine methyl ester (L-NAME) in a model of experimental renal I/R injury. Methods: Male Wistar rats were used in our experiments, and renal I/R injury was achieved after a 30-min occlusion of the bilateral renal artery followed by a 60-min period of reperfusion. Renal function including nitrite plus nitrate excretion and hemodynamics in reperfused kidneys were measured in the presence and absence of L-NAME. Results: Intravenous application of L-NAME (5 mg/kg body weight) resulted in a marked reduction of urine flow, renal plasma flow (0.7±0.3 ml/min), and the glomerular filtration rate (0.1±0.01 ml/min), but a significant increase in NO excretion (FENOx, 67.9±10.5%). In addition we found after L-NAME injection a significant increase of the fractional excretion of sodium (FENa, 49.3±7.7%) and lithium (FELi, 70.2±1.6%), as well as the renal vascular resistance compared with animals with renal I/R but non-treated with L-NAME (P<0.001). Furthermore, we observed a high correlation between FENOx and FELi (r2=0.80, P<0.01). Conclusion: Our results suggest that renal excretion of NO derivatives is not influenced by NO production during renal I/R injury, although NO contributes to the tubular transport capacity in the ischemia/reperfused kidney.
- Subjects
NITRIC-oxide synthases; ISCHEMIA; NITROGEN excretion; RENAL artery; BLOOD circulation disorders; HEMODYNAMICS
- Publication
Langenbeck's Archives of Surgery, 2002, Vol 386, Issue 7, p518
- ISSN
1435-2443
- Publication type
Article
- DOI
10.1007/s00423-001-0256-8