We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Virulence genotype and phenotype of two clinical isolates of Arcobacter butzleri obtained from patients with different pathologies.
- Authors
Baztarrika, Itsaso; Salazar-Sánchez, Adrián; Hernaez Crespo, Silvia; López Mirones, José Israel; Canut, Andrés; Alonso, Rodrigo; Martínez-Ballesteros, Ilargi; Martinez-Malaxetxebarria, Irati
- Abstract
The surge in human arcobacteriosis has increased interest in determining the mechanisms involved in the pathogenesis of Arcobacter butzleri. Here, genomic analyses and in vitro Caco-2 infection, motility, urease and antimicrobial susceptibility testing (AST) assays were used to characterise the virulence and antimicrobial resistance (AMR) determinants of strains HC-1, isolated from a patient with travellers’ diarrhoea, and HC-2, isolated from another with pruritus. AMR determinants conferring resistance to tetracycline (tetO, present in both genomes) and to ampicillin and amoxicillin–clavulanic acid (bla3, present in HC-2) were identified. The same determinants associated with flagellum, chemotaxis, adhesion and invasion were detected in both, but HC-1 lacked eight flagellar genes. The urease cluster was only present in HC-1. Motility and urease tests confirmed the genetic differences between strains, but no genetic marker related to the inability of HC-2 to adhere and invade was identified. This inability could be conditioning the patient’s pathology.
- Publication
Archives of Microbiology, 2023, Vol 205, Issue 12, p1
- ISSN
0302-8933
- Publication type
Article
- DOI
10.1007/s00203-023-03709-3