We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Nuclear Envelope Retention of LINC Complexes Is Promoted by SUN-1 Oligomerization in the Caenorhabditis elegans Germ Line.
- Authors
Daryabeigi, Anahita; Alexander Woglar; Baudrimont, Antoine; Silva, Nicola; Paouneskou, Dimitra; Vesely, Cornelia; Rauter, Manuel; Penkner, Alexandra; Jantsch, Michael; Jantsch, Verena
- Abstract
SUN (Sad1 and UNC-84) and KASH (Klarsicht, ANC-1, and Syne homology) proteins are constituents of the inner and outer nuclear membranes. They interact in the perinuclear space via C-terminal SUN-KASH domains to form the linker of nucleoskeleton and cytoskeleton (LINC) complex thereby bridging the nuclear envelope. LINC complexes mediate numerous biological processes by connecting chromatin with the cytoplasmic force-generating machinery. Here we show that the coiled-coil domains of SUN-1 are required for oligomerization and retention of the protein in the nuclear envelope, especially at later stages of female gametogenesis. Consistently, deletion of the coiled-coil domain makes SUN-1 sensitive to unilateral force exposure across the nuclear membrane. Premature loss of SUN-1 from the nuclear envelope leads to embryonic death due to loss of centrosome-nuclear envelope attachment. However, in contrast to previous notions we can show that the coiled-coil domain is dispensable for functional LINC complex formation, exemplified by successful chromosome sorting and synapsis in meiotic prophase I in its absence.
- Subjects
NUCLEAR membranes; OLIGOMERIZATION; CAENORHABDITIS elegans; GAMETOGENESIS; ANTIBODY diversity; NUCLEAR matrix
- Publication
Genetics, 2016, Vol 203, Issue 2, p733
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.116.188094