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- Title
Genome-wide effects of the antimicrobial peptide apidaecin on translation termination in bacteria.
- Authors
Mangano, Kyle; Florin, Tanja; Xinhao Shao; Klepacki, Dorota; Chelysheva, Irina; Ignatova, Zoya; Yu Gao; Mankin, Alexander S.; Vázquez-Laslop, Nora
- Abstract
Biochemical studies suggested that the antimicrobial peptide apidaecin (Api) inhibits protein synthesis by binding in the nascent peptide exit tunnel and trapping the release factor associated with a terminating ribosome. The mode of Api action in bacterial cells had remained unknown. Here genome-wide analysis reveals that in bacteria, Api arrests translating ribosomes at stop codons and causes pronounced queuing of the trailing ribosomes. By sequestering the available release factors, Api promotes pervasive stop codon bypass, leading to the expression of proteins with C-terminal extensions. Api-mediated translation arrest leads to the futile activation of the ribosome rescue systems. Understanding the unique mechanism of Api action in living cells may facilitate the development of new medicines and research tools for genome exploration.
- Subjects
STOP codons; PROTEIN binding; GENOMICS; PROTEIN synthesis; GENETIC translation; RIBOSOMES; CATHELICIDINS
- Publication
eLife, 2020, p1
- ISSN
2050-084X
- Publication type
Article
- DOI
10.7554/eLife.62655