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- Title
Genetic and serological analyses of elongation factor EF-Tu of paulownia witches’-broom phytoplasma (16SrI-D).
- Authors
Wang, J.; Zhu, X.-P.; Gao, R.; Lin, C.-L.; Li, Y.; Xu, Q.-C.; Piao, C.-G.; Li, X.-D.; Li, H.-F.; Tian, G.-Z.
- Abstract
This study determined the tuf gene sequence of the phytoplasma specific to paulownia witches’-broom from Nanyang, China (hereby designated PaWB-Ny). The PaWB-Ny tuf gene was 1185 nucleotides in length and confirmed that the phytoplasma belongs to subgroup 16SrI-D of aster yellows. Three characteristic GTP-binding protein motifs were identified based on the peptide deduced from the tuf gene sequence. Results suggested that the elongation factor EF-Tu was localized in the cytoplasm and lacked hydrophobic transmembrane domains. Antibodies against PaWB-Ny EF-Tu were prepared by rabbit immunization with glutathione-S-transferase (GST)-tagged EF-Tu fusion protein expressed in Escherichia coli. EF-Tu exhibited a molecular weight of ∼43 kDa and was detected in PaWB-infected paulownia plants by western blot analysis. Indirect enzyme-linked immunosorbent assays (ELISA) and dot blotting analyses were performed with freezing and thawing treatments during antigen preparation. Dilution of extracts to an appropriate scale significantly reduced non-specific reactions. The resultant PaWB EF-Tu antibody reacted with antigens from plants infected with periwinkle virescence and chinaberry tree witches’-broom phytoplasmas, but not those infected with jujube witches’-broom or bishopwood witches’-broom phytoplasma. The EF-Tu was characteristically localized within the phytoplasmal cytoplasm of infected plant phloem tissues.
- Subjects
NANYANG Shi (China); CHINA; PHYTOPLASMA diseases; PAULOWNIA; CARRIER protein genetics
- Publication
Plant Pathology, 2010, Vol 59, Issue 5, p972
- ISSN
0032-0862
- Publication type
Article
- DOI
10.1111/j.1365-3059.2010.02316.x