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- Title
Allele-specific patterns of the mouse parathyroid hormone-related protein: influences on cell adhesion and migration.
- Authors
Benelli, Roberto; Peissel, Bernard; Manenti, Giacomo; Gariboldi, Manuela; Vanzetto, Christian; Albini, Adriana; Dragani, Tommaso A
- Abstract
The mouse parathyroid hormone-like hormone PthlhPro and PthlhThr variants are linked with susceptibility and resistance to skin carcinogenesis of Car-S and Car-R mice, respectively, and with in vitro effects (Oncogene, 19: 5324-5328, 2000). We have identified an additional Pthlh variant, consisting of Thr and three amino-acid changes in the C-terminus (PthlhSerAspTyr), carried by an evolutionarily distant Mus spretus (SPRET/Ei) inbred strain. When transfected into NCI-H520 tumor cells, this PthlhSerAspTyr variant did not stimulate tumor growth in nude mice. Analysis of cell adhesion, migration, and invasion patterns of PthlhPro-, PthlhThr-, and PthlhSerAspTyr-transfected NCI-H520 cells revealed a 1.5-fold decrease in adhesion efficiency on both collagen type I and Matrigel, and a 5-6-fold increase in migration capability in PthlhPro transfectants as compared to nontransfected, vector-transfected, PthlhThr-, or PthlhSerAspTyr-transfected cells. These findings suggest that the cancer modifier effects of the mouse Pthlh gene are mediated by differential cell adhesion and migration effects of PTHrP variants.Oncogene (2003) 22, 7711-7715. doi:10.1038/sj.onc.1207088
- Subjects
CARCINOGENESIS; AMINO acids; CELL adhesion; CELLS; COLLAGEN
- Publication
Oncogene, 2003, Vol 22, Issue 49, p7711
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1207088