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- Title
Integrin α(v)β₃ as a PET imaging biomarker for osteoclast number in mouse models of negative and positive osteoclast regulation.
- Authors
Zheleznyak A; Wadas TJ; Sherman CD; Wilson JM; Kostenuik PJ; Weilbaecher KN; Anderson CJ; Zheleznyak, Alexander; Wadas, Thaddeus J; Sherman, Christopher D; Wilson, Jessica M; Kostenuik, Paul J; Weilbaecher, Katherine N; Anderson, Carolyn J
- Abstract
<bold>Purpose: </bold>The goal of this study was to determine the specificity of ⁶⁴Cu-CB-TE2A-c(RGDyK) (⁶⁴Cu-RGD) for osteoclast-related diseases, such as Paget's disease or rheumatoid arthritis.<bold>Procedures: </bold>C57BL/6 mice were treated systemically with osteoprotegerin (OPG) for 15 days or RANKL for 11 days to suppress and stimulate osteoclastogenesis, respectively. The mice were then imaged by positron emission tomography/computed tomography using ⁶⁴Cu-RGD, followed by determination of serum TRAP5b and bone histology. Standard uptake values were determined to quantify ⁶⁴Cu-RGD in bones and other tissues.<bold>Results: </bold>Mice treated with OPG showed decreased bone uptake of ⁶⁴Cu-RGD at 1, 2, and 24 h post-injection of the tracer (p < 0.01 for all time points) compared to vehicle controls, which correlated with a post-treatment decrease in serum TRAP5b. In contrast, mice treated with RANKL showed significantly increased bone uptake at 2 h post-injection of (⁶⁴Cu-RGD (p < 0.05) compared to the vehicle control group, corresponding to increased serum TRAP5b and OC numbers as determined by bone histology.<bold>Conclusions: </bold>These data demonstrate that ⁶⁴Cu-RGD localizes to areas in bone with increased osteoclast numbers and support the use of ⁶⁴Cu-RGD as an imaging biomarker for osteoclast number that could be used to monitor osteoclast-related pathologies and their treatments.
- Publication
Molecular Imaging & Biology, 2012, Vol 14, Issue 4, p500
- ISSN
1536-1632
- Publication type
journal article
- DOI
10.1007/s11307-011-0512-4