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- Title
Clinical Relevance of LC3B, CXCL10, and Bcl-2 in Breast Cancer.
- Authors
Youssry, Sara A.; Hussein, Amina E.; El-Sheredy, Amel G.; Ramadan, Rabie; El-Sheredy, Heba G.
- Abstract
Autophagy is a highly conserved catabolic process, and its dysfunction has been associated with a variety of human diseases including cancer. The role of autophagy in cancer is complex showing a double-edged sword activity. The chemokine ligand CXCL10 has divergent roles in tumors, either promoting or inhibiting tumor progression. In addition, chemokines appear to modulate senescence and cell survival. This study is aimed at exploring the complex role of autophagic marker microtubuleassociated protein light chain 3B (LC3B) and chemokine CXCL10 among breast cancer patients with a focus on understanding the relation between LC3B and the anti-apoptotic marker Bcl-2. The study was conducted on sixty females who were classified into forty-five breast cancer patients with different stages and fifteen healthy females as the control group. Venous blood samples were obtained for the quantification of LC3B mRNA transcripts using real time PCR technique. The serum was separated for the detection of CXCL10 and Bcl-2 levels using enzyme-linked immunosorbent assay (ELISA). The results showed a significant increase in the mean of LC3B expression, CXCL10 and Bcl2 concentrations in patients. Moreover, the LC3B expression and Bcl2 level were negatively associated with age, tumor stage, lymph-node involvement, and vascular invasion, while a positive association was observed between CXCL10 and both tumor stage and vascular invasion. Furthermore, a positive correlation was observed between LC3B expression and the Bcl2 level. The current study concludes that both LC3B and CXCL10 may act as potential prognostic indicators for breast cancer patients. In addition, the modulation of autophagy and apoptosis could be promising targets for the treatment of breast cancer.
- Subjects
BREAST cancer; ENZYME-linked immunosorbent assay; CELLULAR aging; CANCER invasiveness; TUMOR classification
- Publication
Jordan Journal of Biological Sciences, 2019, Vol 12, Issue 4, p471
- ISSN
1995-6673
- Publication type
Article