We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A Rapid, Amplification-Free, and Sensitive Diagnostic Assay for Single-Step Multiplexed Fluorescence Detection of MicroRNA.
- Authors
Jin, Zongwen; Geißler, Daniel; Qiu, Xue; Wegner, K. David; Hildebrandt, Niko
- Abstract
The importance of microRNA (miRNA) dysregulation for the development and progression of diseases and the discovery of stable miRNAs in peripheral blood have made these short-sequence nucleic acids next-generation biomarkers. Here we present a fully homogeneous multiplexed miRNA FRET assay that combines careful biophotonic design with various RNA hybridization and ligation steps. The single-step, single-temperature, and amplification-free assay provides a unique combination of performance parameters compared to state-of-the-art miRNA detection technologies. Precise multiplexed quantification of miRNA-20a, -20b, and -21 at concentrations between 0.05 and 0.5 n M in a single 150 μL sample and detection limits between 0.2 and 0.9 n M in 7.5 μL serum samples demonstrate the feasibility of both high-throughput and point-of-care clinical diagnostics.
- Subjects
FLUORESCENCE microscopy; MICRORNA; GENETIC regulation; DISEASE progression; NUCLEIC acid regulatory sequences
- Publication
Angewandte Chemie International Edition, 2015, Vol 54, Issue 34, p10024
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201504887