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- Title
Pathogenesis of the <em>Platinum</em> (<em>c<sup>P</sup></em>) Mutation, a Model for Oculocutaneous Albinism.
- Authors
Orlow, Seth J.; Lamoreux, M. Lynn; Pifko-Hirst, Sharon; Zhou, Bao-Kang
- Abstract
The <em>platinum</em> mutation at the C (<em>albino</em>) locus in the mouse is a potential model for oculocutaneous albinism in humans other than type IA (tyrosinase-negative) albinism. Although tissues from mice homozygous for the mutation display substantial tyrosinase activity, cutaneous and ocular pigmentation is severely restricted in affected animals. Using specific antipeptide antisera, we demonstrate that ocular extracts from wild-type mice contain two isoforms of tyrosinase bearing either the amino-terminal PEP5 epitope or the carboxy-terminal PEP7 epitope. The latter isoform participates in a high-molecular-weight complex detectable on sucrose density gradients. In <em>platinum</em> mice, antiserum to the PEP7 epitope fails to recognize any protein species, and the high-molecular-weight form of tyrosinase is not detectable. Our results support a key role for this high-molecular- weight complex in melanogenesis, and suggest that mutations that interfere with the ability of tyrosinase to participate in a multimeric complex may be a cause of oculocutaneous albinism in people.
- Subjects
ALBINOS &; albinism; PLATINUM; PIGMENTATION disorders; PHENOL oxidase; MICE; EPITOPES
- Publication
Journal of Investigative Dermatology, 1993, Vol 101, Issue 2, p137
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12363621