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- Title
Inactivation of dengue, chikungunya, and Ross River viruses in platelet concentrates after treatment with ultraviolet C light.
- Authors
Faddy, Helen M.; Fryk, Jesse J.; Prow, Natalie A.; Watterson, Daniel; Young, Paul R.; Hall, Roy A.; Tolksdorf, Frank; Sumian, Chryslain; Gravemann, Ute; Seltsam, Axel; Marks, Denese C.
- Abstract
<bold>Background: </bold>Arboviruses, including dengue (DENV 1-4), chikungunya (CHIKV), and Ross River (RRV), are emerging viruses that are a risk for transfusion safety globally. An approach for managing this risk is pathogen inactivation, such as the THERAFLEX UV-Platelets system. We investigated the ability of this system to inactivate the above mentioned arboviruses.<bold>Study Design and Methods: </bold>DENV 1-4, CHIKV, or RRV were spiked into buffy coat (BC)-derived platelet (PLT) concentrates in additive solution and treated with the THERAFLEX UV-Platelets system at the following doses: 0.05, 0.1, 0.15, and 0.2 J/cm(2) (standard dose). Pre- and posttreatment samples were taken for each dose, and the level of viral infectivity was determined.<bold>Results: </bold>At the standard ultraviolet C (UVC) dose (0.2 J/cm(2) ), viral inactivation of at least 4.43, 6.34, and 5.13 log or more, was observed for DENV 1-4, CHIKV, and RRV, respectively. A dose dependency in viral inactivation was observed with increasing UVC doses.<bold>Conclusions: </bold>Our study has shown that DENV, CHIKV, and RRV, spiked into BC-derived PLT concentrates, were inactivated by the THERAFLEX UV-Platelets system to the limit of detection of our assay, suggesting that this system could contribute to the safety of PLT concentrates with respect to these emerging arboviruses.
- Subjects
DENGUE; CHIKUNGUNYA; BLOOD platelets; PATHOGENIC microorganisms; BLOOD transfusion; BLOOD platelet transfusion; FLAVIVIRUSES; DOSE-response relationship (Radiation); RNA viruses; ULTRAVIOLET radiation; VIRUS inactivation; CHIKUNGUNYA virus
- Publication
Transfusion, 2016, Vol 56, Issue 6pt2, p1548
- ISSN
0041-1132
- Publication type
journal article
- DOI
10.1111/trf.13519