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- Title
MiR-7, miR-9 and miR-375 contribute to effect of Exendin-4 on pancreatic β-cells in high-fat-diet-fed mice.
- Authors
Chang Guo; Yi-Qiong Sun; Qiang Li; Jin-Chao Zhang; Guo, Chang; Sun, Yi-Qiong; Li, Qiang; Zhang, Jin-Chao
- Abstract
<bold>Purpose: </bold>The purpose of this study was to test whether glucagon-like peptide-1 (GLP-1) receptor activation preserved pancreatic β-cells via the regulation of microRNAs and target genes in high-fat-diet-fed mice.<bold>Methods: </bold>C57BL/6 male mice were simultaneously treated with high-fat-diet (HFD) and GLP-1 analogue, Exendin-4 (Ex-4) (3 μg/kg/day or 30 μg/kg/day), i.p. or vehicle, for consecutive 13 weeks. Fasting blood glucose, postprandial blood glucose, ΔI30/ΔG30, HOMA-IR and HOMA-% β were measured in each group. Pancreatic β-cell mass was assessed by immunohistochemistry. The expression of miRNAs and related downstream genes were investigated using quantitative real-time PCR.<bold>Results: </bold>Thirteen weeks of Ex-4 treatment significantly reduced body weight and food intake in HFD-fed mice (P.
- Subjects
MICRORNA; GLUCAGON-like peptide 1; HIGH-fat diet; EXENDINS; PANCREATIC beta cells; TREATMENT of diabetes; RAPAMYCIN
- Publication
Clinical & Investigative Medicine, 2018, Vol 41, Issue 1, pE16
- ISSN
0147-958X
- Publication type
journal article
- DOI
10.25011/cim.v41i1.29459