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- Title
Synaptic scaffolding molecule interacts with Axin.
- Authors
Hirabayashi, Susumu; Nishimura, Wataru; Iida, Junko; Kansaku, Ai; Kishida, Shosei; Kikuchi, Akira; Tanaka, Noriaki; hata, Yutaka
- Abstract
Synaptic scaffolding molecule (S-SCAM) is a synaptic protein that consists of PDZ domains, a guanylate kinase domain, and WW domains. It interacts with N-methyl- d-aspartate receptor subunits, neuroligin, and β-catenin. Here, we identified Axin as a novel binding partner of S-SCAM. Axin was co-immunoprecipitated with S-SCAM from rat brain, detected in the post-synaptic density fraction in rat brain subcellular fractionation, and partially co-localized with S-SCAM in neurons. The guanylate kinase domain of S-SCAM directly bound to the GSK3β-binding region of Axin. S-SCAM formed a complex with β-catenin and Axin, but competed with GSK3β for Axin-binding. Thereby, S-SCAM inhibited the Axin-mediated phosphorylation of β-catenin by GSK3β.
- Subjects
NEURAL transmission; NEUROTRANSMITTERS; PROTEINS; ORGANIC compounds; ASPARTATE aminotransferase; NEUROCHEMISTRY
- Publication
Journal of Neurochemistry, 2004, Vol 90, Issue 2, p332
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2004.02497.x