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- Title
Individual culture leads to decreased blastocyst formation but does not affect pregnancy outcomes in the setting of a single, vitrified-warmed euploid blastocyst transfer.
- Authors
Glatthorn, Haley N.; Hanson, Brent M.; Kim, Julia G.; Herlihy, Nola S.; Klimczak, Amber M.; Hong, Kathleen H.; Seli, Emre; Scott Jr, Richard T.
- Abstract
Purpose: To evaluate embryology and pregnancy outcomes following individual and group embryo culture in the setting of contemporary laboratory practices and freeze-all cycles. Methods: Patients underwent ovarian stimulation followed by intracytoplasmic sperm injection (ICSI). Embryos proceeded through individual culture and then underwent preimplantation genetic testing for aneuploidy (PGT-A) via trophectoderm biopsy. In a subsequent cycle, participants underwent single embryo transfer of a vitrified-warmed, euploid embryo. Outcomes were compared to controls undergoing group culture during the same time frame. The Mann-Whitney U test and logistic regression models were utilized. Results: Outcomes were assessed for 144 patients whose embryos underwent individual culture and 449 controls whose embryos underwent group culture. There were no significant differences in fertilization rates between groups (81.7% for individual culture vs. 84.1% for group culture, p = 0.22). However, individual culture was associated with a decreased rate of blastocyst formation compared to group culture (43.5% vs. 48.5%, p < 0.01). Following single, vitrified-warmed euploid blastocyst transfer, there were no significant differences between individual culture and group culture, respectively, in rates of positive βhCG (81.9% vs. 81.5%, p = 0.91), sustained implantation (63.9% vs. 65.0%, p = 0.80), biochemical miscarriage (16.7% vs. 12.3%, p = 0.18), or clinical miscarriage (1.4% vs. 4.2%, p = 0.13). Conclusion: While individual culture appears to negatively impact the rate of usable blastocyst formation compared to group culture, there were no significant differences in pregnancy outcomes following transfer of a single, vitrified-warmed euploid blastocyst.
- Subjects
BLASTOCYST; PREGNANCY outcomes; INTRACYTOPLASMIC sperm injection; MANN Whitney U Test; INDUCED ovulation; HUMAN embryology; EMBRYO transfer; HUMAN in vitro fertilization
- Publication
Journal of Assisted Reproduction & Genetics, 2021, Vol 38, Issue 8, p2157
- ISSN
1058-0468
- Publication type
Article
- DOI
10.1007/s10815-021-02252-8