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- Title
The Artificial Evolution of an Enzyme by Random Mutagenesis: The Development of Formaldehyde Dehydrogenase.
- Authors
Fujii, Yasuyo; Yamasaki, Yoshiaki; Matsumoto, Masahiro; Nishida, Hiroyuki; Hada, Megumi; Ohkubo, Katsutoshi
- Abstract
Describes the artificial evolution of a unique variant of glutathione independent formaldehyde dehydrogenase of Pseudomonas putida obtained by random mutagenesis. Use of the polymerase chain reaction; Catalytic efficiency for formaldehyde; Optimum pHs and temperatures of the mutant and the wild-type enzyme.
- Subjects
ALDEHYDE dehydrogenase; FORMALDEHYDE; GLUTATHIONE; PSEUDOMONAS; MUTAGENESIS; POLYMERASE chain reaction
- Publication
Bioscience, Biotechnology & Biochemistry, 2004, Vol 68, Issue 8, p1722
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1271/bbb.68.1722