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- Title
Nocardia lasii sp. nov., a novel actinomycete isolated from the cuticle of an ant ( Lasius fuliginosus L).
- Authors
Liu, Chongxi; Bai, Lu; Ye, Lan; Zhao, Junwei; Yan, Kai; Xiang, Wensheng; Wang, Xiangjing
- Abstract
A novel actinomycete, designated strain 3C-HV12, was isolated from the cuticle of an ant ( Lasius fuliginosus L) and characterised using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain 3C-HV12 belongs to the genus Nocardia with high sequence similarities to Nocardia soli DSM 44488 (99.2 %) and Nocardia cummidelens R89 (99.2 %), and phylogenetically clustered with these two species and Nocardia ignorata DSM 44496 (98.8 %), Nocardia salmonicida JCM 4826 (98.8 %), Nocardia fluminea S1 (98.8 %), Nocardia coubleae OFN N12 (98 %) and Nocardia camponoti 1H-HV4 (97.4 %). The morphological and chemotaxonomic properties of the strain are also consistent with those of members of the genus Nocardia. The strain was observed to form extensively branched substrate hyphae which fragmented into rod-shaped and non-motile elements. The cell wall was found to contain meso-diaminopimelic acid and the whole cell sugars were identified as arabinose and galactose. The predominant menaquinone was identified as MK-8(H). The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were identified as C 10-methyl, C and Cω7c. Mycolic acids were found to be present. A combination of DNA-DNA hybridisation experiments and phenotypic tests were carried out between strain 3C-HV12 and its phylogenetically closely related strains, which further clarified their relatedness and demonstrated that 3C-HV12 could be distinguished from these strains. Therefore, the strain is concluded to represent a novel species of the genus Nocardia, for which the name Nocardia lasii sp. nov. is proposed. The type strain is 3C-HV12 (=DSM 100525 = CGMCC 4.7279).
- Subjects
NOCARDIA; ACTINOBACTERIA; CUTICLE; ANT anatomy; SKIN microbiology; BACTERIA phylogeny
- Publication
Antonie van Leeuwenhoek, 2016, Vol 109, Issue 11, p1513
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-016-0753-y