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- Title
Effect of low-level laser therapy on irradiated parotid glands--study in mice.
- Authors
Dossena Acauan, Monique; Neutziling Gomes, Ana Paula; Braga-Filho, Aroldo; de Figueiredo, Maria Antonia Zancanaro; Cherubini, Karen; Gonçalves Salum, Fernanda
- Abstract
The objective of this study was to evaluate the effect of low-level laser therapy (LLLT) on radiotherapyinduced morphological changes and caspase-3 immunodetection in parotids of mice. Forty-one Swiss mice were divided into control, radiotherapy, 2- and 4-J laser groups. The experimental groups were exposed to ionizing radiation in a single session of 10 Gy. In the laser groups, a GaAlAs laser (830 nm, 100 mW, 0.028 cm2, 3.57 W/cm2) was used on the region corresponding to the parotid glands, with 2-J energy (20 s, 71 J/cm2) or 4 J (40 s, 135 J/cm2) per point. LLLT was performed immediately before and 24 h after radiotherapy. One point was applied in each parotid gland. The animals were euthanized 48 h or 7 days after radiotherapy and parotid glands were dissected for morphological analysis and immunodetection of caspase-3. There was no significant difference between groups in the immunodetection of caspase-3, but the laser groups had a lower percentage compared to the radiotherapy group. LLLT promoted the preservation of acinar structure, reduced the occurrence of vacuolation, and stimulated parotid gland vascularization. Of the two LLLT protocols, the one using 4 J of energy showed better results.
- Subjects
PAROTID glands; LASER therapy; IONIZING radiation; RADIOTHERAPY; MORPHOLOGY
- Publication
Journal of Biomedical Optics, 2015, Vol 20, Issue 10, p108002-1
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.JBO.20.10.108002