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- Title
Structure and gene cluster of the O-antigen of Escherichia coli O165 containing 5-N-acetyl- 7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid.
- Authors
Senchenkova, Sof'ya N.; Yuanyuan Zhang; Perepelov, Andrei V.; Xi Guo; Shashkov, Alexander S.; Weintraub, Andrej; Bin Liu; Widmalm, Göran; Knirel, Yuriy A.
- Abstract
Upon mild acid degradation of the lipopolysaccharide of Escherichia coli O165, the O-polysaccharide chain was cleaved at the glycosidic linkage of 5-N-acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid (Pse5Hb7Ac). Analysis of the resulting linear tetrasaccharide and alkali-treated lipopolysaccharide by ¹H/13C 1D and 2D nuclear magnetic resonance spectroscopy enabled elucidation of the following structure of the O-polysaccharide: →8)-α-Psep5Hb7Ac-(2→6)-β-D-Galp-(1→4)-β-D-Gl?p-(1→3)-α-DGl?pNAc-(1→. The β-D-Galp-(1→4)-β-D-Gl?p-(1→3)-D-Gl?pNAc structural element is also present in the O-polysaccharide of E. coli O82. The content of the O-antigen gene cluster of E. coli O165 was found to be consistent with the O-polysaccharide structure established. Functions of proteins encoded in the gene cluster, including enzymes involved in the Pse5Hb7Ac biosynthesis and glycosyltransferases, were putatively assigned by comparison with sequences in available databases.
- Subjects
CHEMICAL structure; ESCHERICHIA coli; POLYSACCHARIDES; NUCLEAR magnetic resonance spectroscopy; MICROBIAL genes
- Publication
Glycobiology, 2016, Vol 26, Issue 4, p335
- ISSN
0959-6658
- Publication type
Article
- DOI
10.1093/glycob/cwv106