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- Title
Global proteomic mapping of alkali stress regulated molecular networks in Helianthus tuberosus L.
- Authors
Zhang, Aiqin; Zang, Wei; Zhang, Xiyang; Ma, Yangyang; Yan, Xiufeng; Pang, Qiuying
- Abstract
Background and aims: Soil salinization with high pH condition is a major abiotic stress to plant growth and crop productivity. Helianthus tuberosus L. is an important stress tolerant plant and can survive in the saline-alkali soil and semiarid areas. The aim of this study is to identify the effect of alkali stress on H. tuberosus through global proteomics analysis and improve understanding of the alkalinity resistance of plants. Methods: H. tuberosus seedlings were exposed to different level alkali stress for 7 days. Protein profiling was quantified by conducting MS-based comparative proteomics analysis. RT-PCR study was carried out to analyze the mRNA expression levels of candidate alkali stress response proteins. Results: The response of H. tuberosus to alkali stress was detected at both physiological and molecular levels. 104 differentially expressed proteins from H. tuberosus leaves response to NaCO treatment were successfully identified. Functional categorization of these identified proteins showed that the accumulation level of proteins involved in glycolysis, TCA cycle, PSI system, ROS scavenging and signal transduction increased under alkali stress. Conclusions: Based on the observation of plant growth and the investigation of molecular regulation, H.tuberosus could resist certain alkali stress by modulating carbohydrate metabolism and redox homeostasis. These findings provide a new sight into the underlying molecular mechanisms of alkali resistance in plant.
- Subjects
PLANT proteomics; PROTEOMICS; MOLECULAR biology; SUNFLOWERS; PROTEIN genetics
- Publication
Plant & Soil, 2016, Vol 409, Issue 1/2, p175
- ISSN
0032-079X
- Publication type
Article
- DOI
10.1007/s11104-016-2945-7