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- Title
三七皂苷R1保护四氯化碳诱导肝纤维化模型大鼠的作用.
- Authors
吴 朕; 马 微; 臧成昊; 刘矿嫔; 刘 伟; 刘 洁; 梁 宇; 李春艳; 陈志明; 茹 金; 樊楚明; 杨金伟; 郭建辉; 李力燕
- Abstract
BACKGROUND: Previous studies have found that panax notoginseng saponins have a certain protective effect on immunological liver injury in mice. OBJECTIVE: To explore the therapeutic effect of notoginsenoside R1 on carbon tetrachloride-induced liver fibrosis in rats. METHODS: Experimental liver fibrosis model was made by carbon tetrachloride in male Sprague-Dawley rats. Then 30 g/L notoginsenoside R1 (60 mg/kg) was given once daily for 4 and 6 weeks in the treatment group. Rats in the control and model group were given distilled water of the same volume. Histopathological observation with hematoxylin-eosin staining and Masson’s trichrome staining was used to evaluate the changes of liver structure and fibrosis degree. The expression of collage type I, α-smooth muscle actin and transforming growth factor-β1 mRNA of hepatic tissue was measured by qRT-PCR method. The experimental protocol was approved by the Animal Experiment Ethics Committee of Kunming Medical University (approval No. KMMU2018018). RESULTS AND CONCLUSION: Liver histopathology showed that notoginsenoside R1 improved the degree of liver fibrosis. The expression levels of collagen type I, α-smooth muscle actin and transforming growth factor-β1 mRNA were reduced significantly in the treatment group compared with the model group (P < 0.05). But there was no significant difference after 4 and 6 weeks of treatment with notoginsenoside R1. Overall findings indicate that notoginsenoside R1 can slow down the progression of carbon tetrachloride-induced liver fibrosis in rats to a certain extent.
- Subjects
TREATMENT effectiveness; MEDICAL ethics committees; CARBON tetrachloride; DISTILLED water; TRANSFORMING growth factors
- Publication
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu, 2020, Vol 24, Issue 26, p4213
- ISSN
2095-4344
- Publication type
Article
- DOI
10.3969/j.issn.2095-4344.2768