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- Title
有氧运动对梗阻性黄疸模型小鼠肝纤维化治疗的机制.
- Authors
彭 瑞; 陈 伟; 毛海峰; 张 宇
- Abstract
BACKGROUND: Hepatocyte apoptosis or necrosis due to cholestasis after obstructive jaundice can induce chronic liver injury. Liver fibrosis is a repairing reaction, which may cause pathological changes, such as liver dysfunction and liver tissue sclerosis. OBJECTIVE: To analyze the relationship between the expression of gene LincRNA-p21 in liver tissue of obstructive jaundice mice and its biological function, and to explore the mechanism of LincRNA-p21 through the Notch pathway improving liver fibrosis in obstructive jaundice mice after aerobic exercise. METHODS: Thirty-five male ICR mice were provided by Hunan Slack Jingda Experimental Animal Co., Ltd., and the study was approved by the Ethics Committee of Hunan Normal University, approval No. 2018-183. All mice were used to construct the obstructive jaundice model with the common bile duct hanged on the abdominal wall. Five mice were randomly selected to testify whether the modeling is successful. The remaining 30 mice were randomly divided into aerobic exercise, model, and control groups. The aerobic exercise group underwent treadmill adaptive training for 1 week. At 1-2 days, the treadmill slop was 0° with the speed of 6 m/min, 20 min/d. At 3-4 days, the treadmill slop was 5° with the speed of 8 m/min, 40 min/d. At 5-6 days, the treadmill slop was 8° with the speed of 10 m/min, 60 min/d. After adaptive training, the treadmill slop was kept at 8° with the speed of 10 m/min, 60 min/d, and 6 d/week. The samples were removed under anesthesia at 7 days to detect each index. RESULTS AND CONCLUSION: (1) Compared with the control group, the serum concentrations of total bilirubin, total bile acid, glutamate pyruvate transaminase, glutamic oxalacetic transaminase and alkaline phosphatase were significantly increased in the model group (P < 0.01). The aerobic exercise group showed different degrees of reduction compared with the model group (P < 0.05, P < 0.01). (2) In the model group, hematoxylin-eosin staining showed the hepatocytes with large-area fibrosis and disordered hepatic cord, vacuolar, degeneration and necrotic symptoms. The fibrosis degree in the aerobic exercise group was lower than that in the model group. (3) Compared with the model group, the expression levels of LincRNA-p21 protein and mRNA in the aerobic exercise and control groups were increased (P < 0.01), and the control group was higher than in the aerobic exercise group. The expression levels of Notch-1, Jagged-1, NICD, and HES-1 protein and mRNA were highest in the model group (P < 0.01), followed by aerobic exercise group. (4) To conclude, aerobic exercise can promote the high expression of LincRNA-p21, thereby inhibiting the Notch pathway to participate in the process of liver fibrosis in mice after obstructive jaundice, and can regulate the repair after liver injury to some extent.
- Publication
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu, 2019, Vol 23, Issue 27, p4369
- ISSN
2095-4344
- Publication type
Article
- DOI
10.3969/j.issn.2095-4344.1387