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- Title
Chromosome mapping of Xenopus tropicalis using the G- and Ag-bands: Tandem duplication and polyploidization of larvae heads.
- Authors
Uehara, Mariko; Haramoto, Yoshikazu; Sekizaki, Hiroyuki; Takahashi, Shuji; Asashima, Makoto
- Abstract
Developmental cytogenetic analyses of Xenopus tropicalis larvae from two origins were performed on stage 27–34 heads treated with colchicine. Standard G-band karyotyping using trypsin and chromosome mapping of 184 bands were examined. Although the main karyotype was 2n = 20, polyploidy (3n = 30 or 4n = 40) and aneuploidy were detected in each individual treated with colchicine, even those treated for only 1 h. The percentage of polyploid karyotypes was 10–20% across the total of measured metaphases. The mean mitotic index was 0.10. Chromosomal breaks and exchanges were detected at the secondary constriction of chromosomes 5 or 6. Ag-band detection showed clearly positive staining at the secondary constriction of chromosome 5, which corresponds to the nucleolar organizer region. Tandem duplication of negative G-bands at the secondary constriction of chromosome 6 and the short arm of chromosome 10 was suggested by this study. X. tropicalis thus provides a good model to study the mechanism and effects of chromosomal abnormalities, gene mapping and tissue specific gene expression in the developmental process.
- Subjects
XENOPUS; POLYPLOIDY; COLCHICINE; KARYOTYPES
- Publication
Development, Growth & Differentiation, 2002, Vol 44, Issue 5, p427
- ISSN
0012-1592
- Publication type
Article
- DOI
10.1046/j.1440-169X.2002.00656.x