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- Title
Polyphenolic extract attenuates fatty acid-induced steatosis and oxidative stress in hepatic and endothelial cells.
- Authors
Vergani, Laura; Vecchione, Giulia; Baldini, Francesca; Grasselli, Elena; Voci, Adriana; Portincasa, Piero; Ferrari, Pier Francesco; Aliakbarian, Bahar; Casazza, Alessandro A.; Perego, Patrizia
- Abstract
Purpose: Phenolic compounds (PC) of virgin olive oil exert several biochemical and pharmacological beneficial effects. Some dietary PC seem to prevent/improve obesity and metabolic-related disorders such as non-alcoholic fatty liver disease (NAFLD). We investigated the possible effects of PC extracted from olive pomace (PEOP) and of the main single molecules present in the extract (tyrosol, apigenin, oleuropein, p-coumaric and caffeic acid) in protecting hepatocytes and endothelial cells against triglyceride accumulation and oxidative stress.Methods: Rat hepatoma and human endothelial cells were exposed to a mixture of oleate/palmitate to mimic the condition of NAFLD and atherosclerosis, respectively. Then, cells were incubated for 24 h in the absence or in the presence of PC or PEOP. Different parameters were evaluated, such as lipid accumulation and oxidative stress-related markers.Results: In hepatic cells, expression of peroxisome proliferator-activated receptors (PPARs) and of stearoyl-CoA desaturase 1 (SCD-1) were assessed as index of lipid metabolism. In endothelial cells, expression of intercellular adhesion molecule-1 (ICAM-1), activation of nuclear factor kappa-B (NF-kB), release of nitric oxide (NO), and wound-healing rate were assessed as index of inflammation.Conclusion: PEOP extract ameliorated hepatic lipid accumulation and lipid-dependent oxidative imbalance thus showing potential applications as therapeutic agent tuning down hepatosteatosis and atherosclerosis.
- Subjects
ANIMAL experimentation; ATHEROSCLEROSIS; CELL adhesion molecules; CELL receptors; ENDOTHELIUM; FATTY liver; NITRIC oxide; PHENOLS; RATS; TRIGLYCERIDES; WOUND healing; DNA-binding proteins; OXIDATIVE stress; PEROXISOME proliferator-activated receptors; IN vitro studies
- Publication
European Journal of Nutrition, 2018, Vol 57, Issue 5, p1793
- ISSN
1436-6207
- Publication type
Article
- DOI
10.1007/s00394-017-1464-5