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- Title
A nano self-assembled artificial peroxidase: spectroscopic and electrochemical investigations.
- Authors
Gao, Yun-Fei; Zhao, Ying-Xue; Yang, Tian; Xiao, Bao-Lin; Zhang, Yu-Shuai; Yang, Xiao-Lu; Hong, Jun; Ghourchian, Hedayatollah; Sheibani, Nader; Moosavi-Movahedi, Ali
- Abstract
A nano-micelle with highly efficient peroxide activity was constructed by self-assembly of sodium dodecyl sulfate micellar, histidine and hematin in 50 mM phosphate buffer at 25 °C. UV-Vis spectrometry methods were utilized for characterization of the nanostructured material or artificial peroxidase (AP). The Michaelis-Menten ( K) and catalytic rate ( k) constants of the AP were obtained to be 5.5 μM and 0.06 s, respectively, in 50 mM phosphate buffer solution at pH 8.0. The catalytic efficiency of AP was evaluated to be 0.011 μM s. The AP was also immobilized on a functional multi-wall carbon nanotubes-gold nanoparticles (AuNPs) nano-complex modified glassy carbon electrode (GCE). The transmission electron microscopy method was utilized for the characterization of the nano-materials. The electron-transfer rate constant ( k) and the apparent Michaelis-Menten constant K of the AP modified GCE were evaluated to be 1.36 s and 0.19 μM, respectively. For a biosensor without a redox protein, the properties of the AP modified GCE were significant and will further benefit from additional studies and improvement.
- Subjects
MOLECULAR self-assembly; PEROXIDASE; ELECTROCHEMICAL analysis; MICELLES; SODIUM dodecyl sulfate; NANOSTRUCTURED materials; MICHAELIS-Menten equation
- Publication
Journal of the Iranian Chemical Society, 2014, Vol 11, Issue 5, p1397
- ISSN
1735-207X
- Publication type
Article
- DOI
10.1007/s13738-014-0410-1