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- Title
Synthesis and Evaluation of Amphiphilic Poly(tetrahydrofuran- b -ethylene oxide) Copolymers for DNA Delivery into Skeletal Muscle.
- Authors
Catherine Pomel; Christian Leborgne; Hervé Cheradame; Daniel Scherman; Antoine Kichler; Philippe Guegan
- Abstract
Abstract Purpose Amphiphilic triblock copolymers such as the pluronic poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) L64 (pEO13-pPO30-pEO13) have been shown to mediate more efficient gene transfer in muscle as compared to naked DNA. We were interested in studying the effect of a chemical change of the central block of pluronic polymers on the transfection activity. Methods We synthesized new amphiphilic copolymers in which the hydrophobic pPO block was replaced by poly(tetrahydrofuran) (pTHF) chains. The resulting triblock pEO–pTHF–pEO polymers have been characterized by NMR and SEC and assayed for in vitro and in vivo gene transfer. Results The animal experiments showed that the new copolymers are able to significantly increase the transfection efficiency of plasmid DNA after intramuscular injection. Conclusions These results indicate that the capacity to enhance plasmid DNA transfection in skeletal muscle is not restricted to pEO–pPO–pEO arrangements.
- Subjects
ETHYLENE oxide; BLOCK copolymers; GENETIC transformation; ETHYLENE synthesis; DRUG delivery systems; TETRAHYDROFURAN; NUCLEAR magnetic resonance
- Publication
Pharmaceutical Research, 2008, Vol 25, Issue 12, p2963
- ISSN
0724-8741
- Publication type
Article
- DOI
10.1007/s11095-008-9698-9