We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion.
- Authors
Shimatani, Zenpei; Kashojiya, Sachiko; Takayama, Mariko; Terada, Rie; Arazoe, Takayuki; Ishii, Hisaki; Teramura, Hiroshi; Yamamoto, Tsuyoshi; Komatsu, Hiroki; Miura, Kenji; Ezura, Hiroshi; Nishida, Keiji; Ariizumi, Tohru; Kondo, Akihiko
- Abstract
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
- Publication
Nature Biotechnology, 2017, Vol 35, Issue 5, p441
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt.3833