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- Title
Induction of a midbrain dopaminergic phenotype in Nurr1-overexpressing neural stem cells by type 1 astrocytes.
- Authors
Wagner, Joseph; Åkerud, Peter; Castro, Diogo S.; Holm, Pontus C.; Canals, Josep M.; Snyder, Evan Y.; Perlmann, Thomas; Arenas, Ernest
- Abstract
The implementation of neural stem cell lines as a source material for brain tissue transplants is currently limited by the ability to induce specific neurochemical phenotypes in these cells. Here, we show that coordinated induction of a ventral mesencephalic dopaminergic phenotype in an immortalized multi-potent neural stem cell line can be achieved in vitro. This process requires both the overexpression of the nuclear receptor Nurr1 and factors derived from local type 1 astrocytes. Over 80% of cells obtained by this method demonstrate a phenotype indistinguishable from that of endogenous dopaminergic neurons. Moreover, this procedure yields an unlimited number of cells that can engraft in vivo and that may constitute a useful source material for neuronal replacement in Parkinson's disease.
- Subjects
CELLULAR therapy; PARKINSON'S disease; TYROSINE in the body
- Publication
Nature Biotechnology, 1999, Vol 17, Issue 7, p653
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/10862