We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Osthole Protects Bone Marrow-Derived Neural Stem Cells from Oxidative Damage through PI3K/Akt-1 Pathway.
- Authors
Yan, Yu-hui; Li, Shao-heng; Li, Hong-yan; Lin, Ying; Yang, Jing-xian
- Abstract
In recent years, neural stem cell (NSC) transplantation has been widely explored as a treatment for neurodegenerative diseases. NSCs are special cells that have some capacity for self-renewal and the potential to differentiate into multiple cell types. However, the inflammatory environment of diseased tissue is not conducive to the survival of transplanted cells. Osthole (Ost) is a principal bioactive component of Fructus Cnidii, Radix Angelicae Pubescentis and other traditional Chinese medicines. Ost has a wide range of pharmacological activities, such as anti-inflammation, immunomodulation, and neuroprotection. In the present study, we assessed the protective effects of Ost on bone marrow-derived-NSCs (BM-NSCs) against injury induced by hydrogen peroxide (HO). BM-NSCs were pre-treated with different doses of Ost and treated with HO. The cell counting kit-8 (CCK-8) method and lactate dehydrogenase (LDH) leakage assay were used to determine cell viability. Using the TUNEL assay and RT-PCR, we evaluated the effect of Ost on cell apoptosis. The results showed that Ost had protective effects against HO-induced cell damage, and the number of apoptotic cells was significantly decreased in the Ost pre-treated groups compared to the HO group. The expression ratio of Bax/Bcl-2 mRNA was also decreased. Furthermore, western blotting was used to analyze levels of proteins related to PI3K/Akt-1 signaling pathway, and results indicated that ost can increase p-Akt and PI3K. Our findings suggested that Ost protects BM-NSCs against oxidative stress injury, and it can be used to improve the inflammatory environment of neurodegenerative diseases so and promote the survival rate of transplanted NSCs.
- Subjects
NEURAL stem cell transplantation; NEURODEGENERATION; BONE marrow cells; PHOSPHOINOSITIDES; IMMUNOREGULATION; OXIDATIVE stress; THERAPEUTICS
- Publication
Neurochemical Research, 2017, Vol 42, Issue 2, p398
- ISSN
0364-3190
- Publication type
Article
- DOI
10.1007/s11064-016-2082-y