We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Identification of Novel α-Synuclein Isoforms in Human Brain Tissue by using an Online NanoLC-ESI-FTICR-MS Method.
- Authors
Öhrfelt, Annika; Zetterberg, Henrik; Andersson, Kerstin; Persson, Rita; Secic, Dzemila; Brinkmalm, Gunnar; Wallin, Anders; Mulugeta, Ezra; Francis, Paul; Vanmechelen, Eugeen; Aarsland, Dag; Ballard, Clive; Blennow, Kaj; Westman-Brinkmalm, Ann
- Abstract
Parkinson's disease (PD) and Dementia with Lewy bodies (DLB) are neurodegenerative diseases that are characterized by intra-neuronal inclusions of Lewy bodies in distinct brain regions. These inclusions consist mainly of aggregated α-synuclein (α-syn) protein. The present study used immunoprecipitation combined with nanoflow liquid chromatography (LC) coupled to high resolution electrospray ionization Fourier transform ion cyclotron resonance tandem mass spectrometry (ESI-FTICR-MS/MS) to determine known and novel isoforms of α-syn in brain tissue homogenates. N-terminally acetylated full-length α-syn (Ac-α-syn) and two N-terminally acetylated C-terminally truncated forms of α-syn (Ac-α-syn and Ac-α-syn) were found. The different forms of α-syn were further studied by Western blotting in brain tissue homogenates from the temporal cortex Brodmann area 36 (BA36) and the dorsolateral prefrontal cortex BA9 derived from controls, patients with DLB and PD with dementia (PDD). Quantification of α-syn in each brain tissue fraction was performed using a novel enzyme-linked immunosorbent assay (ELISA).
- Subjects
PROTEIN analysis; PRECIPITIN reaction; PARKINSON'S disease; DEMENTIA; NEURODEGENERATION; CEREBROSPINAL fluid; MASS spectrometry; LIQUID chromatography; ELECTROSPRAY ionization mass spectrometry
- Publication
Neurochemical Research, 2011, Vol 36, Issue 11, p2029
- ISSN
0364-3190
- Publication type
Article
- DOI
10.1007/s11064-011-0527-x