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- Title
A single, multiplex analysis for all relevant activating NRAS gene mutations using heteroduplex generators.
- Authors
Belli, Carolina; Bowen, Derrick J.; De Brasi, Carlos; Larripa, Irene
- Abstract
We describe a multiplex polymerase chain reaction (PCR)-based test that detected all relevant NRAS activating mutations using a single PCR followed directly by electrophoresis. The test uses a Universal Heteroduplex Generator (UHG) to detect exon-2 (codon 61) NRAS mutations in multiplex with an UHG for exon-1 (codons 12 and 13). The method differentiated all 19 relevant mutations in these exons and showed a mutation independent sensitivity of approximately 6%. The sensitive, specific detection of all NRAS activating mutations using this single rapid test represents a minimum workload and could be applied readily for large-scale screening and for routine analysis.
- Subjects
RENIN-angiotensin system; POLYMERASE chain reaction; EXONS (Genetics); ONCOGENES; GENETIC mutation; ELECTROPHORESIS
- Publication
British Journal of Haematology, 2004, Vol 126, Issue 4, p602
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1111/j.1365-2141.2004.05079.x