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- Title
Preparation and Hydroxyl Radical-scavenging Effects of Tea Polysaccharides Metal Complex.
- Authors
WANG Yuan-feng; JIN Zheng-yu; WEI Xin-lin
- Abstract
A kind of tea polysaccharides ,coded as ATPS, which was mainly acid polysaccharides were obtained from the coarse green tea which was purified by anion exchange resin D315. ATPS formed metal complexes when chelated with Fe3+ and Ca2+. It showed that the coordination degrees was different in which the amount of ATPS-Ca(II) complex was much more than that of ATPS-Fe(III). FT-IṚ scan showed that ATPS was mainly composed of secondary hydroxyl and carboxyl by the way of C-O coordination. The chemiluminescence(CL) method was used to investigate the free radical scavenging activity of the polysaccharides with different structures. It showed that the HO · scavenging activities of ATPS and its complexes were much more than the O2 scavenging activities. HO · scavenging activity of ATPS-Ca(II) was much weaker than ATPS, but that of ATPS-Fe(III) had almost the same with ATPS. On the one hand, this might be that the chelating degrees of ATPS with Ca2+ was much more that that with Fe3+ and HO · scavenging activities was probably related with the number and occupation of the positions for chelating metal ions; on the other hand, this might be concerned with the less activities of tea polysaccharides reacted with O2.
- Subjects
HYDROXYL group; POLYSACCHARIDES; GREEN tea; ANIONS; IRON; CALCIUM
- Publication
Natural Product Research & Development, 2009, Vol 21, Issue 3, p382
- ISSN
1001-6880
- Publication type
Article