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- Title
Oncolytic adenovirus expressing interleukin-18 induces significant antitumor effects against melanoma in mice through inhibition of angiogenesis.
- Authors
Zheng, J.-N.; Pei, D.-S.; Mao, L.-J.; Liu, X.-Y.; Sun, F.-H.; Zhang, B.-F.; Liu, Y.-Q.; Liu, J.-J.; Li, W.; Han, D.
- Abstract
It has been shown that interleukin 18 (IL-18) exerts antitumor activity. In this study, we investigated whether oncolytic adenovirus-mediated gene transfer of IL-18 could induce strong antitumor activity. A tumor-selective replicating adenovirus expressing IL-18 (ZD55-IL-18) was constructed by insertion of an IL-18 expression cassette into the ZD55 vector, which is based on deletion of the adenoviral E1B 55-kDa gene. It has been shown that ZD55-IL-18 exerted a strong cytopathic effect and significant apoptosis in tumor cells. ZD55-IL-18 significantly decreased vascular endothelial growth factor and CD34 expression in the melanoma cells. Treatment of established tumors with ZD55-IL-18 showed much stronger antitumor activity than that induced by ZD55-EGFP (enhanced green fluorescent protein) or Ad-IL-18. These data indicated that oncolytic adenovirus expressing IL-18 could exert potential antitumor activity through inhibition of angiogenesis and offer a novel approach to melanoma therapy.
- Subjects
ADENOVIRUSES; INTERLEUKINS; NEOVASCULARIZATION; MELANOMA; GENETIC transformation
- Publication
Cancer Gene Therapy, 2010, Vol 17, Issue 1, p28
- ISSN
0929-1903
- Publication type
Article
- DOI
10.1038/cgt.2009.38