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- Title
Identification of extended-spectrum β-lactamase and quinolone resistance genes in Escherichia coli isolated from hospital wastewater from central India.
- Authors
Diwan, Vishal; Chandran, Salesh P.; Tamhankar, A. J.; Stålsby Lundborg, Cecilia; Macaden, Ragini
- Abstract
Objectives To investigate the presence of antibiotic resistance genes (ARGs), related to commonly used β-lactams and quinolones, in Escherichia coli present in hospital wastewater in central India. Methods Cefotaxime- and ciprofloxacin-resistant E. coli isolates from hospital-associated wastewater samples were collected from two tertiary care hospitals in the Ujjain district of India during 2008–09. The presence of blaCTX-M, blaTEM, blaSHV, qnrA, qnrB, qnrS, aac(6′)-Ib-cr and qepA genes was detected by PCR and sequencing. Results Twenty-five E. coli isolates were extended-spectrum β-lactamase (ESBL) positive. blaCTX-M-15 and blaTEM-1 genes were identified in 21 and 16 ESBL-positive isolates by PCR, respectively. Amongst 30 fluoroquinolone-resistant E. coli isolates, aac(6′)-Ib-cr was detected in 27 isolates, qnrA in 1 isolate, qnrB in 2 isolates and qepA in 3 isolates. Conclusions blaCTX-M-15, blaTEM-1, aac(6′)-Ib-cr, qnrA, qnrB and qepA genes are present in E. coli occurring in hospital wastewater in central India.
- Subjects
INDIA; DRUG resistance in microorganisms; BETA lactam antibiotics; QUINOLONE antibacterial agents; DRUG resistance in bacteria; ESCHERICHIA coli; SEWAGE microbiology; HOSPITALS; MICROBIAL genetics
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2012, Vol 67, Issue 4, p857
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dkr564