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- Title
Alterations of Ultrastructural and Fission/Fusion Markers in Hepatocyte Mitochondria From Mice Following Calorie Restriction With Different Dietary Fats.
- Authors
Khraiwesh, Husam; López-Domínguez, José A.; López-Lluch, Guillermo; Navas, Plácido; de Cabo, Rafael; Ramsey, Jon J.; Villalba, José M.; González-Reyes, José A.
- Abstract
We analyzed ultrastructural changes and markers of fission/fusion in hepatocyte mitochondria from mice submitted to 40% calorie restriction (CR) for 6 months versus ad-libitum-fed controls. To study the effects of dietary fat under CR, animals were separated into three CR groups with soybean oil (also in controls), fish oil, and lard. CR induced differential changes in hepatocyte and mitochondrial size, in the volume fraction occupied by mitochondria, and in the number of mitochondria per hepatocyte. The number of cristae per mitochondrion was significantly higher in all CR groups compared with controls. Proteins related to mitochondrial fission (Fis1 and Drp1) increased with CR, but no changes were detected in proteins involved in mitochondrial fusion (Mfn1, Mfn2, and OPA1). Although many of these changes could be attributed to CR regardless of dietary fat, changing membrane lipid composition by different fat sources did modulate the effects of CR on hepatocyte mitochondria.
- Subjects
ULTRASTRUCTURE (Biology); LIVER cells; MITOCHONDRIA; LABORATORY mice; LOW-calorie diet; MEMBRANE lipids; CONTROL groups; FISH oils
- Publication
Journals of Gerontology Series A: Biological Sciences & Medical Sciences, 2013, Vol 68, Issue 9, p1023
- ISSN
1079-5006
- Publication type
Article
- DOI
10.1093/gerona/glt006