We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Intense Foxp3<sup>+</sup>CD25<sup>+</sup> regulatory T-cell infiltration is associated with high-grade cutaneous squamous cell carcinoma and counterbalanced by CD8<sup>+</sup>/Foxp3<sup>+</sup>CD25<sup>+</sup> ratio.
- Authors
Azzimonti, B.; Zavattaro, E.; Provasi, M.; Vidali, M.; Conca, A.; Catalano, E.; Rimondini, L.; Colombo, E.; Valente, G.
- Abstract
Background Recent reports have revealed the therapeutic potential of cell-mediated immunity in neoplasms such as cutaneous squamous cell carcinoma ( SCC). Objectives To define the antigenic coexpression of regulatory T cells (Tregs) and plasmacytoid dendritic cells ( pDCs) and assess the CD8+/Foxp3+CD25+ cell ratio at peritumoral and intratumoral levels in order to investigate a correlation with the aggressiveness of SCC tumours. Methods We evaluated the content and distribution of Foxp3+CD25+ Treg and CD123+ pDC infiltration and assessed CD8+/Foxp3+CD25+ cell ratio at peritumoral and intratumoral levels in 40 SCCs (20 well-differentiated, G1; and 20 moderately to poorly differentiated, G2-G3) to investigate a correlation with their aggressiveness. We determined the profiles of Tregs and CD123+ cells; immunostained for CD4, CD8, CD123, interleukin (IL)-1 and transforming growth factor (TGF)-β1; and unequivocally double stained for Foxp3CD25. Results Peritumorally, CD4, CD8 and Foxp3 expression showed no difference between the two groups. CD123+ cells were fewer in G2-G3 ( P = 0·0005), while Foxp3+ CD25+ cells were more numerous ( P = 0·0005). The Foxp3+ CD25+/Foxp3+ ratio was higher in G2-G3 cases ( P = 0·0005), confirming the trend in this group of activated T lymphocytes towards total Treg Foxp3+ cells, while the CD8+/Foxp3+ CD25+ ratio was higher in G1 ( P = 0·0005). Intratumorally, CD4+ and CD8+ cells infiltrated G2-G3 ( P = 0·048) more than G1 ( P = 0·004), whereas almost all cells were CD123 negative. Regarding Foxp3 CD25, TGF-β1 and IL-10, they were less expressed in G1, whereas they were positive in G2-G3 ( P < 0·05). The CD8+/Foxp3+ CD25+ ratio was similar to that observed in peritumoral infiltration. Conclusions Our data suggest that intratumoral recruitment of Tregs, high expression of TGF-β1 and IL-10, almost negative CD123+, and a low CD8+/Foxp3+ CD25+ T-cell ratio may contribute to the aggressiveness of cutaneous SCC, as already evidenced for other solid tumours.
- Subjects
CELLULAR immunity; TUMOR surgery; SQUAMOUS cell carcinoma; T cells; INTERLEUKINS; TRANSFORMING growth factors
- Publication
British Journal of Dermatology, 2015, Vol 172, Issue 1, p64
- ISSN
0007-0963
- Publication type
Article
- DOI
10.1111/bjd.13172