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- Title
Molecular emm typing of Bulgarian macrolide-resistant Streptococcus pyogenes isolates.
- Authors
Muhtarova, Adile; Mihova, Kalina; Markovska, Rumyana; Mitov, Ivan; Kaneva, Radka; Gergova, Raina
- Abstract
Group A streptococcus (GAS) is a human pathogen causing a broad range of infections, linked with global morbidity and mortality. Macrolide resistance rates vary significantly in different parts of the world. Driving factors of the emergence and spread of resistant clones are not clearly understood. We investigated 102 macrolide-resistant GAS strains collected during the period 2014–2018 from various clinical specimens from Bulgarian patients. Strains were characterized by the presence of mefA/mefE, ermA , and ermB using polymerase chain reaction and sequencing for mefA/mefE. Resistant strains were studied by emm sequence typing and emm -cluster system. Most prevalent emm types among the macrolide-resistant GAS strains were emm 28 (22.55%), emm 12 (17.65%), and emm 4 (16.66%). Almost all (87.25%) of the macrolide-resistant isolates harboring ermB were emm 28. The isolates that carried ermA were predominantly emm 12 (38.24%) and emm 77 (38.24%), with fewer emm 89 (23.53%). The isolates harbored predominantly mefE (49 isolates) and only 9 strains carried mefA. The most prevalent emm clusters among the GAS isolates were E4 (40.20%), A-C4 (17.65%), and E1 (16.66%). The study's results suggest that dissemination of specific clones in GAS population may also be the reason for the increasing macrolide-resistance rate in our country.
- Subjects
STREPTOCOCCUS pyogenes; STREPTOCOCCUS; POLYMERASE chain reaction
- Publication
Acta Microbiologica et Immunologica Hungarica, 2020, Vol 67, Issue 1, p14
- ISSN
1217-8950
- Publication type
Article
- DOI
10.1556/030.66.2019.033