We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Streptococcus pneumoniae using Ultracentrifugation, Ultrafiltration and Iodixanol Gradient Fractionation.
- Authors
Zaipul Anuar, Nurul Fathiyah binti; Desa, Mohd Nasir Mohd; Hussaini, Jamal; Eng Hwa Wong; Mariappan, Vanitha; Vellasamy, Kumutha Malar; P., Navindra Kumari
- Abstract
Extracellular membrane vesicles (EMVs) are membranous structures that are excreted by gram-positive bacteria. These vesicles are involved in a multitude of biological functions, essential for adaptability to the environment, cellular component exchange, antigen and virulence factor distribution, and infection transmission. Recently, bacterial EMVs have gained attention due to their potential as highly effective vaccine targets. However, extraction of EMVs from bacterial cells has been difficult. Briefly, in this study, the extracellular membrane vesicles (EMVs) of Streptococcus pneumoniae were extracted from its common serotypes of (6A, 14, 19A, 19F, and 23F) by using ultracentrifugation, ultrafiltration and iodixanol gradient fractionation. The extracted EMVs was validated by viewing its morphology by transmission electron microscope (TEM). In this study, the six serotypes used showed to released extracellular vesicles, albeit in varying numbers and sizes. (22 nm -250 nm). The extracellular vesicles measure 20-250 nm in diameter and contain various biologically active proteins that are required for bacterial nutrient acquisition, biofilm formation, and pathogenesis. Bacterial EMVs are non-viable component of the bacteria that acts as an antigen, hence able to induce host immune response. This suggests it to be a potential vaccine candidate for this bacterium. In this study, the success of extracting EMVs from S. pneumoniae using the modified method has now opened a path to study better drug targets for S. pneumoniae.
- Subjects
ULTRACENTRIFUGATION; STREPTOCOCCUS pneumoniae; EXTRACELLULAR vesicles; TRANSMISSION electron microscopes; CELL anatomy; GRAM-positive bacteria; ULTRAFILTRATION
- Publication
Current Trends in Biotechnology & Pharmacy, 2023, Vol 17, p49
- ISSN
0973-8916
- Publication type
Article